abstract：:Performances of serological parallel and serial testing algorithms were analyzed using a combination of three ELISA and three rapid tests for the confirmation of HIV infection. Each was assessed individually for their sensitivity and specificity on a blinded panel of 769 retrospective sera of known HIV status. Western...

journal_title：Journal of virological methods

authors： Zeh C,Oyaro B,Vandenhoudt H,Amornkul P,Kasembeli A,Bondo P,Mwaengo D,Thomas TK,Hart C,Laserson KF,Ondoa P,Nkengasong JN

更新日期：2011-09-01 00:00:00

abstract：:Classical swine fever (CSF) is a highly contagious disease, causing severe economic losses in the pig industry worldwide. Vaccination of pigs with lapinized Chinese vaccines is still practised in some regions of the world, where the virus is enzootic, in order to prevent and control the disease. However, a single real...

journal_title：Journal of virological methods

authors： Liu L,Xia H,Everett H,Sosan O,Crooke H,Meindl-Böhmer A,Qiu HJ,Moennig V,Belák S,Widén F

更新日期：2011-08-01 00:00:00

abstract：:Five methods for the RNA detection of rabies virus were directly compared in this study. These included conventional nucleic acid sequence-based amplification with electrochemiluminescence (NASBA-ECL) assay, reverse transcription (RT)-heminested (hn) polymerase chain reaction (PCR) and TaqMan real-time RT-PCR using pr...

journal_title：Journal of virological methods

authors： Wacharapluesadee S,Phumesin P,Supavonwong P,Khawplod P,Intarut N,Hemachudha T

更新日期：2011-08-01 00:00:00

abstract：:Avian influenza virus (AIV) is an enveloped virus with segmented RNA that belongs to the Orthomyxoviridae. Recently, avian influenza virus isolates have not only posed a significant threat to the poultry industry but also serious public health concerns. The full-length viral hemagglutinin (HA), neuraminidase (NA) or b...

journal_title：Journal of virological methods

authors： Tsai SM,Chiang YC,Chin LT,Liu HJ,Wang CY

更新日期：2011-08-01 00:00:00

abstract：:Treatment of chronic hepatitis C is associated with varying success rates, substantial medical costs and serious side effects. Several host polymorphisms have been identified near the IL28B gene, of which the homozygous rs12979860 CC was found to be associated with significantly favourable treatment outcome. To determ...

journal_title：Journal of virological methods

authors： Kovanda A,Poljak M

更新日期：2011-07-01 00:00:00

abstract：:Comparative evaluation of Abbott RealTime and Innogenetics INNO-LiPA on alternately processed formalin-fixed, paraffin-embedded specimens of 31 cervical cancers and 31 uterine myomas showed complete agreement in the detection of 14 assay-common HPV genotypes and partial genotyping of HPV-16 and HPV-18. The tissue prep...

journal_title：Journal of virological methods

authors： Kocjan BJ,Seme K,Poljak M

更新日期：2011-07-01 00:00:00

abstract：:Porcine endogenous retroviruses (PERV) represent a risk for xenotransplantation using pig cells, tissues or organs. PERV-A and PERV-B are present in the genome of all pigs and both infect human cells in vitro. PERV-C infects only pig cells and it is integrated in the genome of most, but not all pigs. Recombinants betw...

journal_title：Journal of virological methods

authors： Kaulitz D,Mihica D,Dorna J,Costa MR,Petersen B,Niemann H,Tönjes RR,Denner J

更新日期：2011-07-01 00:00:00

abstract：:Human coronavirus NL63 was identified in 2004 in the Netherlands. Due to the high prevalence and world-wide distribution of this pathogen, it is essential to develop a sensitive and specific detection assay suitable for use in a routine diagnostic laboratory. Techniques based on PCR or real-time PCR are laborious and ...

journal_title：Journal of virological methods

authors： Pyrc K,Milewska A,Potempa J

更新日期：2011-07-01 00:00:00

abstract：:A novel assay for the pan-serotypic detection of foot-and-mouth disease virus (FMDV) was designed using a 5' conjugated minor groove binder (MGB) probe real-time RT-PCR system. This assay targets the 3D region of the FMDV genome and is capable of detecting 20 copies of a transcribed RNA standard. The linear range of t...

journal_title：Journal of virological methods

authors： McKillen J,McMenamy M,Reid SM,Duffy C,Hjertner B,King DP,Bélak S,Welsh M,Allan G

更新日期：2011-06-01 00:00:00

abstract：:West Nile virus (WNV) is an emerging zoonotic pathogen with a wide range of hosts, including birds, horses and humans. The development and evaluation of the performance of a new enzyme-linked immunosorbent assay (ELISA) are described for rapid detection of WNV-specific antibodies in samples originating from an extensi...

journal_title：Journal of virological methods

authors： Sotelo E,Llorente F,Rebollo B,Camuñas A,Venteo A,Gallardo C,Lubisi A,Rodríguez MJ,Sanz AJ,Figuerola J,Jiménez-Clavero MÁ

更新日期：2011-06-01 00:00:00

abstract：:Classical swine fever is a highly contagious viral disease that causes significant economic losses in pig production on a global scale. The rapid dissemination of the virus and the variability of the clinical signs merit the development of swift and accurate classical swine fever virus (CSFV) detection methods, which ...

journal_title：Journal of virological methods

authors： Pérez LJ,Díaz de Arce H,Tarradas J,Rosell R,Perera CL,Muñoz M,Frías MT,Nuñez JI,Ganges L

更新日期：2011-06-01 00:00:00

abstract：:A simple and fast sap-direct RT-PCR (reverse transcription-polymerase chain reaction) for the rapid detection of 3 viroids of the genus Coleviroid is presented. The templates for cDNA synthesis were obtained directly from the sap of coleus using a pipettor, a common tool in molecular biology laboratories, and 3 coleus...

journal_title：Journal of virological methods

authors： Jiang D,Wu Z,Xie L,Sano T,Li S

更新日期：2011-06-01 00:00:00

abstract：:Resistance to oseltamivir in pandemic (H1N1) 2009 influenza A virus is linked to an amino acid change from histidine (H) to tyrosine (Y) at position 275 in the neuraminidase protein (NA). A real-time one step RT-PCR assay using single nucleotide polymorphism (SNP) probes was developed to detect this mutation in respir...

journal_title：Journal of virological methods

authors： Wong S,Pabbaraju K,Wong A,Fonseca K,Drews SJ

更新日期：2011-05-01 00:00:00

abstract：:Lactate dehydrogenase-elevating virus (LDV) can infect transplantable mouse tumors or xenograft tumors in mice through LDV-contaminated mouse biological materials, such as Matrigel, or through mice infected with LDV. LDV infects specifically mouse macrophages and alters immune system and tumor phenotype. The tradition...

journal_title：Journal of virological methods

authors： Liu H,Bockhorn J,Dalton R,Chang YF,Qian D,Zitzow LA,Clarke MF,Greene GL

更新日期：2011-05-01 00:00:00

abstract：:Multiple studies have examined the use of oral fluids in modified serum-based assays aiming to replace serum in antibody detection for hepatitis A. However, the reliable detection of HAV immunity in oral fluid requires an extremely sensitive assay; most immunoassays designed for serum antibody determination lack suffi...

journal_title：Journal of virological methods

authors： Tourinho RS,Amado LA,Villar LM,Sampaio DV,Moraes AC,Rodrigues do Ó KM,Gaspar AM,de Paula VS

更新日期：2011-05-01 00:00:00

abstract：:Three anti-H5 influenza virus monoclonal antibody (mAb) clones, IFH5-26, IFH5-115 and IFH5-136, were obtained by immunising a BALB/C mouse with inactivated A/duck/Hokkaido/Vac-1/04 (H5N1). These mAbs were found to recognise specifically the haemagglutinin (HA) epitope of the influenza H5 subtypes by western blotting w...

journal_title：Journal of virological methods

authors： Miyagawa E,Kogaki H,Uchida Y,Fujii N,Shirakawa T,Sakoda Y,Kida H

更新日期：2011-05-01 00:00:00

abstract：:Asthma and chronic obstructive pulmonary disease exacerbations are associated with human rhinovirus (HRV) lung infections for which there are no current effective antiviral therapies. To date, HRV infectivity of cells in vitro has been measured by a variety of biochemical and immunological methods. This paper describe...

journal_title：Journal of virological methods

authors： Phillips T,Jenkinson L,McCrae C,Thong B,Unitt J

更新日期：2011-05-01 00:00:00

abstract：:Water is one major pathways by which the white spot syndrome virus (WSSV) pathogen enters aquaculture facilities. This paper describes the production and use of a capacitive biosensor for the quantitative detection of as little as 1copy/μl of WSSV in shrimp pond water. A glutathione-S-transferase tag for white spot bi...

journal_title：Journal of virological methods

authors： Samanman S,Kanatharana P,Chotigeat W,Deachamag P,Thavarungkul P

更新日期：2011-04-01 00:00:00

abstract：:The propagation of Taura syndrome virus (TSV) in primary hemocyte culture of Pacific white shrimp (Penaeus vannamei) was investigated. Purified TSV was inoculated into a 24 h old primary hemocyte culture and the development of cytopathic effects was monitored. The cell morphology started changing within 6 h post-inocu...

journal_title：Journal of virological methods

authors： George SK,Kaizer KN,Betz YM,Dhar AK

更新日期：2011-03-01 00:00:00

abstract：:Commercial enzyme-linked immunosorbent assay (ELISA) kits for the determination of the in vitro potency of recombinant hepatitis B vaccines, which detect hepatitis B surface antigen (HBsAg), have been used frequently as an alternative for traditional in vivo potency tests. With the constant need for validation procedu...

journal_title：Journal of virological methods

authors： Costa CI,Delgado IF,da Costa JA,de Carvalho RF,Mouta Sda S Jr,Vianna CO,de Moraes MT

更新日期：2011-03-01 00:00:00

abstract：:Norovirus is associated commonly with human sewage and is responsible for numerous cases of waterborne and foodborne gastroenteritis every year. Assays using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) have been developed for norovirus, however, accurate detection and quantitation remain pro...

journal_title：Journal of virological methods

authors： Gregory JB,Webster LF,Griffith JF,Stewart JR

更新日期：2011-03-01 00:00:00

abstract：:Peste des petits ruminants virus (PPRV) causes a devastating disease of small ruminants present across much of Africa and Asia. Recent surveillance activities and phylogenetic analyses have suggested that the virus is an emerging problem as it is now being detected in areas previously free of the disease. As such, the...

journal_title：Journal of virological methods

authors： Batten CA,Banyard AC,King DP,Henstock MR,Edwards L,Sanders A,Buczkowski H,Oura CC,Barrett T

更新日期：2011-02-01 00:00:00

abstract：:The hemagglutination inhibition (HI) assay has been the main method used to investigate immune responses to vaccination against influenza H1N1 (2009) virus. However microneutralization tests (MNT) have been shown to be more sensitive and more specific. In this study, the three methods of choice: (i) the HI assay, (ii)...

journal_title：Journal of virological methods

authors： Grund S,Adams O,Wählisch S,Schweiger B

更新日期：2011-02-01 00:00:00

abstract：:A monoclonal antibody (Mab)-based blocking ELISA was developed for the detection of serum neutralizing antibodies to porcine circovirus type 2 (PCV2). The Mab with neutralizing activity, which was produced by immunizing a recombinant capsid protein of PCV2 expressed in insect cells, was used as the detector antibody. ...

journal_title：Journal of virological methods

authors： Huang L,Lu Y,Wei Y,Guo L,Liu C

更新日期：2011-01-01 00:00:00

abstract：:The gene encoding the capsid protein in ORF1 of the genome of infectious myonecrosis virus (IMNV) (GenBank AY570982) was amplified into three parts named CP-N (nucleotides 2248-3045), CP-I (nucleotides 3046-3954) and CP-C (nucleotides 3955-4953). The CP-N fragment was inserted into expression vector pTYB1 while CP-I a...

journal_title：Journal of virological methods

authors： Kunanopparat A,Chaivisuthangkura P,Senapin S,Longyant S,Rukpratanporn S,Flegel TW,Sithigorngul P

更新日期：2011-01-01 00:00:00

abstract：:Previous work demonstrated recently the adaptation of the Escherichia coli biotin ligase BirA - biotin acceptor sequence (BAS) labeling system to produce human immunodeficiency virus type 1 viruses with biotinylated integrase (NLXIN(B)) and matrix (NLXMA(B)) proteins (Belshan et al., 2009). This report describes the c...

journal_title：Journal of virological methods

authors： Belshan M,Matthews JM,Madson CJ

更新日期：2011-01-01 00:00:00

abstract：:Early detection of resistant mutations of hepatitis B virus (HBV) is important for patients on nucleos(t)ide analog therapy. An assay based on the PCR-Invader technology was developed to detect resistant mutations with high sensitivity. The assay specifically detects mutations at codons 180, 181, 184, 202, 204, and 25...

journal_title：Journal of virological methods

authors： Tadokoro K,Suzuki F,Kobayashi M,Yamaguchi T,Nagano M,Egashira T,Kumada H

更新日期：2011-01-01 00:00:00

abstract：:Classical swine fever (CSF) is one of the most severe diseases of pigs, and can cause immense economic losses. Real-time reverse transcription polymerase chain reaction (rRT-PCR) can be used as a sensitive and specific method for detection of Classical swine fever virus (CSFV). Different published protocols are used r...

journal_title：Journal of virological methods

authors： Leifer I,Blome S,Beer M,Hoffmann B

更新日期：2011-01-01 00:00:00

abstract：:Maize chlorotic mottle virus (MCMV) causes corn lethal necrosis disease, and can be transmitted through infected maize seeds. It remains a challenge to detect this virus in the seeds to prevent its introduction and infection. For this purpose, a real-time TaqMan RT-PCR procedure for efficient detection of MCMV was dev...

journal_title：Journal of virological methods

authors： Zhang Y,Zhao W,Li M,Chen H,Zhu S,Fan Z

更新日期：2011-01-01 00:00:00

abstract：:Antibodies to non-structural protein 1 (NS1) of West Nile virus (WNV) have been used to differentiate WNV infection from infection by serologically cross-reactive flaviviruses, including Japanese encephalitis virus (JEV), in horses. However, since the inactivated West Nile (WN) vaccine has been reported to induce NS1 ...

journal_title：Journal of virological methods

authors： Kitai Y,Kondo T,Konishi E

更新日期：2011-01-01 00:00:00

abstract：:A nanoparticle-based immunoassay was developed for the rapid and sensitive detection of avian influenza virus (AIV). In this method, AIV-specific pentabody (pVHH3B) was conjugated to magnetic nanoparticles (MNPs) and used to capture AIV. Gold nanoparticles (GNPs), labelled with the anti-AIV mouse monoclonal antibody 3...

journal_title：Journal of virological methods

authors： Mu B,Huang X,Bu P,Zhuang J,Cheng Z,Feng J,Yang D,Dong C,Zhang J,Yan X

更新日期：2010-11-01 00:00:00

abstract：:Prion disorders occur when endogenous prion protein (PrP(C)) undergoes a conformational change from a predominantly α-helix-rich structure to an insoluble β-sheet-rich structure (PrP(Sc)). The resulting PrP(Sc) then in some way facilitates the progressive transformation of nearby PrP(C) to PrP(Sc). In time this result...

journal_title：Journal of virological methods

authors： McMahon A,Han S,Walker I

更新日期：2010-11-01 00:00:00

abstract：:Laboratory analysis of blood specimens is an increasingly important tool for rapid diagnosis and control of therapy. So, miniaturization of test systems is needed, but reduced specimens might impair test quality. For rapid detection and quantitation of HBV DNA, the COBAS(®) AmpliPrep/COBAS(®) TaqMan(®) HBV test has pr...

journal_title：Journal of virological methods

authors： Berger A,Gohl P,Stürmer M,Rabenau HF,Nauck M,Doerr HW

更新日期：2010-11-01 00:00:00

abstract：:Rift Valley fever virus (RVFV) is an arthropod-borne pathogen that often results in severe morbidity and mortality in both humans and livestock. As its geographic range continues to expand, it presents a real threat to naïve populations around the world by accidental introduction (e.g., the result of increased travel)...

journal_title：Journal of virological methods

authors： Mandell RB,Koukuntla R,Mogler LJ,Carzoli AK,Holbrook MR,Martin BK,Vahanian N,Link CJ,Flick R

更新日期：2010-11-01 00:00:00

abstract：:Double-stranded RNA (dsRNA) is in many cases the only available template for molecular and diagnostic studies of RNA viruses. A novel mycovirus with a five dsRNAs segmented-genome served as a model system for the amplification and cloning of dsRNA segments using several PCR-based methods. Sequences obtained by the cla...

journal_title：Journal of virological methods

authors： Darissa O,Willingmann P,Adam G

更新日期：2010-11-01 00:00:00

abstract：:Ultra-deep pyrosequencing (UDPS) of targeted amplicons allows to determine a large number of individual sequence reads from a single PCR product, and this approach is thus extremely valuable for analysis of mixed viral populations. A mixture of genetically distinct DNA templates, however, may lead to the generation of...

journal_title：Journal of virological methods

authors： Görzer I,Guelly C,Trajanoski S,Puchhammer-Stöckl E

更新日期：2010-10-01 00:00:00

abstract：:The availability of whole genome sequencing has contributed to many aspects of dengue research, and its use in dengue virus (DENV) surveillance for early epidemic warning has been proposed. Methods to sequence the genomes of individual dengue serotypes have been described previously, but no single method is known to b...

journal_title：Journal of virological methods

authors： Christenbury JG,Aw PP,Ong SH,Schreiber MJ,Chow A,Gubler DJ,Vasudevan SG,Ooi EE,Hibberd ML

更新日期：2010-10-01 00:00:00

abstract：:A flow cytometry-based immuno-titration titer assay was established to determine infectious unit (IU) and transducing unit (TU) of modified vaccinia Ankara (MVA) virus vectors. This titration method enumerates infected cells by measuring the expression of viral protein for IU and transgene protein for TU in individual...

journal_title：Journal of virological methods

authors： Li Z,Ling L,Liu X,Laus R,Delcayre A

更新日期：2010-10-01 00:00:00

abstract：:Japanese encephalitis (JE) is caused by the Japanese encephalitis virus (JEV). It is a major public health problem in Asia. JEV infects swine which results in fatal encephalitis, abortion and stillbirth in pregnant sow, and hypospermia in boars. Swine is a viral amplifier, and thus plays a critical role in JEV transmi...

journal_title：Journal of virological methods

authors： Li Y,Hou L,Ye J,Liu X,Dan H,Jin M,Chen H,Cao S

更新日期：2010-09-01 00:00:00

abstract：:Avian influenza viruses (AIVs) of the H9N2 subtype are a major economic problem in the poultry industry in Israel. Most field isolates from the last decade differ significantly from H9N2 isolates from Europe and the USA, rendering published detection methods inadequate. This study aimed to develop a real-time TaqMan((...

journal_title：Journal of virological methods

authors： Ben Shabat M,Meir R,Haddas R,Lapin E,Shkoda I,Raibstein I,Perk S,Davidson I

更新日期：2010-09-01 00:00:00